10/23/2023 0 Comments Sputum gram negative rodsĪntibiotic-resistance genes are nowadays a constant presence not only in the hospital environment but are also more and more demonstrated in various ecosystems. The results are not constant, for example, some authors do not find a positive effect of tea tree oil on supragingival plaque. There are many studies about antibacterial effects of EOs, mainly on supragingival plaque. Terpinen-4-ol found in many EOs are demonstrated to act synergistically with chemotherapeutic agents in digestive malignancies. Although the specific mechanisms of their components are not deciphered, many studies demonstrated certain biological activity of some phytochemicals. Essential oils (EOs) from natural products proved their positive effects in various clinical conditions. Thus, they correctly guessed that concentrated lotions/oils are not only more efficient but easy to use and to store. Since ancient times, people have had an intuitive feeling that some spices have real benefits not only as air fresheners but also in that era they were the only therapeutic option for many diseases. Therefore, it is of highest interest in finding alternative methods for preventing contamination with multidrug-resistant strains. Antibiotic-resistant patterns of bacterial isolates from nosocomial infections are continuously changed. Like other beta-lactamines, the carbapenems are included in preferred treatment regimens for various infectious diseases. Nosocomial infections caused by Gram-negative bacteria resistant to carbapenems, mostly multiple-resistant, represent a challenge due to limited treatment options. Improving the management of bacterial pneumonia led to guidelines for use by health-care personnel. In patients in intensive care units (ICUs), the ventilator-associated pneumonia (VAP) is a constant concern for practitioners. Class B carbapenemases are most widely distributed, metallo-beta-lactamases being present in bacteria as well in Archaea.Įnterobacteriaceae and nonfermenters Gram-negative bacilli are increasingly isolated from lower respiratory tract infections. Carbapenemases show striking similarities inside each beta-lactamase class (A, D, and B), no matter their origin-environmental organisms or clinical isolates. In an attempt to evaluate the magnitude of environmental spreading of the carbapenemase genes, 40 carbapenemase sequences of different organisms were compared. Among them, Thymus vulgaris, Eugenia caryophyllata, Origanum vulgare, Melaleuca alternifolia and Aniba rosaeodora essential oils proved to be efficient against Acinetobacter baumannii carbapenem-resistant strain and Escherichia coli ATCC 25922. Attempts to find complementary options for the respiratory contamination and treatment of carbapenem-resistant Gram-negative bacillary pneumonia led us to test the susceptibility of 21 essential oils. Morphological characters of bacterial cells provide important clues about the nature of infection, prior to bacterial isolation and identification. Bacteriological diagnosis, starting with accurate Gram smear performed from properly collected specimens and ending with antibiotic susceptibility testing, is essential. Limited treatment options are the main problems for physicians and clinical microbiologists who have to face such clinical cases. Carbapenem-resistant Gram-negative organisms are increasingly isolated from lower respiratory tract infections.
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